ERK介导的炎性反应参与肾缺血再灌注损伤 |
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引用本文: | 张炯,张颖,肖芳,兰小勤,江潮,杨娟,余冲,裴广畅,李月强,李俊华.ERK介导的炎性反应参与肾缺血再灌注损伤[J].国外医学:分子生物学分册,2013(6):336-339. |
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作者姓名: | 张炯 张颖 肖芳 兰小勤 江潮 杨娟 余冲 裴广畅 李月强 李俊华 |
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作者单位: | [1]华中科技大学同济医学院附属同济医院肾内科,武汉市430030 [2]华中科技大学同济医学院附属同济医院消化内科,武汉市430030 |
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基金项目: | 国家自然科学基金(No.81100498,81100264) |
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摘 要: | 目的研究小鼠肾缺血再灌注损伤的发病机制。方法建立小鼠肾缺血再灌注损伤模型。12只雄性C57BL/6随机分为2个组(n=6),分别为假手术组(Sham),肾缺血再灌注损伤模型组(IRI)。IRI组血管夹夹闭左肾动脉,置于32℃温箱后1h松开血管夹,去除右肾。Sham组操作同上,但不夹闭左肾动脉。再灌注24h后处死小鼠,收集血清和肾脏标本。测定血清肌酐(Cr)和血尿素氮(BUN)。PAS染色后显微镜下观察肾脏形态学变化,Western印迹分析ERK、p-ERK的表达,PCR检测MCP-1、IFN-γ。结果与假手术组(Sham)相比,IRI组血清肌酐、血尿素氮明显升高,病理检查可见肾脏内肾小管上皮细胞明显肿胀坏死、蛋白管型形成明显,还可观察到炎性细胞浸润明显增加。ERK、p-ERKWestern印迹结果PCR显示MCP-1、TNF-α也明显上调,但ERK表达不变。结论在肾缺血再灌注中,ERK激活介导的炎性后府可能参与了肾扣伤。
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关 键 词: | 肾缺血再灌注损伤 ERK 炎症 |
ERK-Mediated Inflammation Is Involved in Renal Ischemia/Reperfu- sion Injury |
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Authors: | ZHANG Jiong ZHANG Ying XIAO Fang LAN Xiaoqin JIANG Chao YANG Juan YU Chong PEI Guangehang LI Yueqiang LI Junhua |
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Institution: | 1 Division of Nephrology, 2Division of Gastroenterology, Tongji Hospital, Huazhong University of Sci- ence and Technology, Wuhan, 430030, China) |
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Abstract: | Objective To explore the mechanism of renal ischemia/reperfusion (I/R) injury in mice. Methods The renal I/R injury- models were established in mice. Twelve male C57BL/6 mice were randomly divided into two groups: sham group and I/R injury (IRI) group. The mice in the IRI group underwent clamping of the renal pedieles, then remained in the 32℃ incubator for 1 hour, and had their right kidneys removed. The mice in the sham group received the same proce- dures except clamping of the renal pedicles. These animals were sacrificed after 24 h of reperfu- sion. The blood samples were collected and measured for the levels of serum creatinine (Cr) and u- rea nitrogen (BUN) . The pathological changes of renal tissues were observed after PAS stai- ning. The mRNA levels of MCP-1 and TNF-α were measured by Q-PCR. The expression of extracel- lular signal-regulated kinase (ERK) and p-ERK was measured by Western blotting. Results The levels of Cr and BUN were significantly increased in the IRI group compared with the sham group. Pathological examination revealed a great number of swollen necrotic renal tubular epithelial cells, the formation of protein casts and increased inflammatory infiltrates in the IRI group. Moreover, the mRNA levels of MCP-land TNF-α, and the expression of p-ERK were signifi- cantly up-regulated in the IRI group. But there was no significant difference in the expression of ERK between the two groups. Conclusions The ERK-mediated inflammation is involved in the renal I/Rinjury. |
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Keywords: | renal ischemia/reperfusion injury inflammation |
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