DREB类转录因子介导的烟草抗非生物胁迫特性研究

检测并分析了转BnDREB1-5基因烟草叶片的持水性能,上、下表皮气孔大小和密度及叶绿素含量,并在自然失水7 h后检测了细胞的离子泄漏状况。结果表明:转基因烟草叶片单位时间内每平方厘米的失水量是野生型烟草叶片的62%;上表皮的气孔大于野生型,而气孔开度小于野生型;野生型烟草叶片上的气孔密度接近转基因烟草的1.5倍;野生型烟草叶片的叶绿素含量比转基因烟草叶片高29%;野生型烟草叶片的质膜相对透性是转基因烟草叶片的1.4倍。

The Tolerance to Abiotic Stresses Mediated by DREB-like Transcription Factors in Nicotiana tabacum

The biological properties on tobacco with 35S:BnDREB1-5 transformation were analyzed which included the holding water capacity,the size and density of upper and lower epidermis stomata,the content of chlorophyll,and plasma membranes permeability after physical drought stressed 7 h.The results indicated that water-holding capacity of 35S:BnDREB1-5 transgenic tobacco leaves was 62% of that of wild-types.The upper epidermis stomata apparatus was bigger in transgenic tobacco than wild-type,but the opening of stoma was smaller in upper epidermis than wild-types.The stomata density was 1.5 times in wild-type leaves as large as in transgenic leaves.The content of chlorophyll was 29% higher in leaves of wild-type than in transgenic leaves,and the plasma membranes permeability were 1.4 times as much as in transgenic tobacco.