The electron transfer in the membranes of endoplasmic reticulum. A quantitative estimation of cytochrome b5 content in the NADPH- and NADH-oxidation chains. |
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Authors: | A I Archakov V M Devichensky |
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Institution: | Central Research Laboratory, N. I. Pirogov Second Medical Institute, Moscow 119435, USSR |
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Abstract: | The phenomenon of induction of the NADPH-specific hydroxylase system by sodium phenobarbital was used to determine the content of cytochrome b5 in each microsomal electron-transfer chain. It turned out that the specific activities of NADPH-dependent reductases and the cytochrome P-450 quantity were increased approximately 1.86 times and the activities of NADH-dependent reductases were somewhat decreased (0.89 times) in microsomes of induced rats. It is assumed that a subfraction of cytochrome b5 included in the NADPH-oxygenase complex is induced together with the other carriers of the chain. The second subfraction of the hemoprotein, in the course of induction, behaves as a typical component of the NADH-oxidizing complex. On the basis of the data obtained, the calculation was made, which showed that the NADPH-oxidation chain contains from to of the total microsomal cytochrome b5 pool. |
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