| 替代宿主增殖松毛虫质型多角体病毒的比较研究 |
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| 引用本文： | 马永平,孟小林,胡蓉,徐进平. 替代宿主增殖松毛虫质型多角体病毒的比较研究[J]. 中国病毒学, 2001, 16(2): 155-160 |
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| 作者姓名： | 马永平 孟小林 胡蓉 徐进平 |
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| 作者单位： | 武汉大学病毒研究所, |
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| 摘 要： | 银纹夜蛾幼虫(Argyrogrammaagnata)对松毛虫CPV(DpCPV)十分敏感，本文对两种不同的宿主增殖松毛虫CPV作了比较，电镜证实用替代宿主增殖的DpCPV与原毒株多角体(CPB)和病毒粒子的形态完全一致。3%PAGE分析二者的RNA图谱基本一致，有大小相同的2.98×10
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| 关 键 词： | 松毛虫；质型多角体病毒；替代宿主；银纹夜蛾；基因组 |
| 文章编号： | 1003-5125(2001)02-0155-06 |
| 修稿时间： | 2000-02-13 |
Comparison of ProducingDendrolimus punctatus Cytoplasmic Polyhedrosis Virus in Substitutive Host Insect |
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| MA Yong-ping,MENG Xiao-lin,HU Rong,XU Jing-ping. Comparison of ProducingDendrolimus punctatus Cytoplasmic Polyhedrosis Virus in Substitutive Host Insect[J]. Virologica Sinica, 2001, 16(2): 155-160 |
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| Authors: | MA Yong-ping MENG Xiao-lin HU Rong XU Jing-ping |
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| Abstract: | Argyrogramma agnata has been selected as a substitutive host insect for producing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus (DpCPV). In our experiment, it is very susceptible to DpCPV. The DpCPV produced in A. agnata is designated Aa-DpCPV. The cytoplasmic polyhedra body (CPB), the virion size and the shape of Aa-DpCPV are same as that of its original DpCPV (DpCPV-W1984). The RNA bands of Aa-DpCPV and DpCPV-W1984 all have 10 RNA segments respectively in 3% PAGE, which molecular weights ranged in size from 2.98×106 to 0.66×106 Dalton. Aa-DpCPV has the same strong toxicity as that of DpCPV-W1984 (from D. punctatus) to D. punctatus (Walker) larva. So it can be applied to the pine caterpillar control. The DpCPV yield in A.agnata is 2.5×108CPB/larva. |
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