Mitochondrial hexokinase activity in a murine hybridoma

Mitochondrially bound hexokinases are often found in cells exhibiting high rates of glycolysis. These enzymes have kinetic and regulatory properties that differ from cytosolic hexokinases. In this work, we present preliminary findings for a mitochondrially bound hexokinase in a murine hybridoma that is relatively insensitive to glucose-6-phosphate inhibition when compared to the cytosolic hexokinase. Additional regulatory data on the effects of glucose-1,6-diphosphate and adenosine diphosphate on both the mitochondrial and cytosolic forms of this enzyme are also presented.