栎生桑黄和忍冬桑黄液体培养过程中发酵液抗氧化能力

桑黄孔菌属Sanghuangporus是药用木生真菌资源中一个重要的属,但是该属仅有少数几个种类被用于人工栽培,且栽培面积较小。此外,桑黄孔菌属中大部分种类的药用功能仍未完全明确。因此,本研究以桑黄孔菌属近期新发表的新种栎生桑黄S. quercicola和关注度较低的忍冬桑黄S. lonicericola为主要研究对象,通过测定它们液体培养过程中第2、4、6、8、10、12和14天的菌丝生物量以及发酵液的粗多糖含量、多酚含量、黄酮含量、抗坏血酸含量、DPPH自由基清除能力、ABTS自由基清除能力、总抗氧化能力、超氧化物歧化酶活性、羟自由基清除能力、超氧阴离子清除能力、铁离子还原能力和亚铁离子螯合能力等12个抗氧化指标,对桑黄的抗氧化能力进行评定。2种桑黄真菌各选取一号菌株,其发酵液均表现出强抗氧化能力。相比之下,栎生桑黄的多糖、抗坏血酸和超氧化物歧化酶含量更高,而忍冬桑黄的多酚和黄酮含量更高。相应的,栎生桑黄和忍冬桑黄在其他一些抗氧化指标上也表现出强弱程度及出现时间的差异。上述研究结果为桑黄孔菌属真菌的药用功能开发提供了新资源,为不同抗氧化代谢产物的分离纯化提供了科学依据。

Antioxidant activities of Sanghuangporus quercicola and S. lonicericola from fermentation broth in liquid cultivation

Sanghuangporus is a genus of important medicinal wood-inhabiting fungi; however, only a few species have been artificially cultivated in a small scale. The medicinal functions of most species are still imperfectly known. In this study, a recently newly described species S. quercicola and an unattractive species S. lonicericola are used as materials for evaluation of antioxidant activity of the fungi. Mycelial biomass and 12 indicative data (polysaccharide content, polyphenol content, flavonoid content, ascorbic acid content, DPPH radical scavenging activity, ABTS radical scavenging activity, total antioxidant capacity, superoxide dismutase activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, ferric ion reducing antioxidant power and ferrous ion chelating ability) of fermentation broths were tested under different duration of liquid cultivation. The result was that fermentation broth of the two species both showed strong antioxidant activities. S. quercicola produced higher content of polysaccharides, ascorbic acid and superoxide dismutase, while the content of polyphenols and flavonoids from S. lonicericola were higher. These results provide new data for medicinal value of Sanghuangporus.