不饱和脂肪酸参与调控金针菇菌柄伸长的潜在分析

金针菇Flammulina filiformis菌柄是产量构成的重要组成部分,也是评价质量的重要指标。菌盖下方0.6-1.5cm是菌柄的伸长区,其他区段不伸长。为了研究菌柄伸长的调控机制,我们采用液相色谱-串联质谱联用（LC-MS/MS）技术对金针菇菌柄的伸长区、过渡区和非伸长区进行检测,结合层次聚类分析（HCA）、t检验等统计学方法对差异代谢物进行研究,并通过KEGG分析代谢物富集情况。结果显示,共有69种代谢物在伸长区、过渡区和非伸长区呈现梯度差异,其中52种代谢物含量为梯度性降低,17种代谢物为梯度性增加。这些差异代谢物主要归属于脂肪酸、氨基酸、核苷酸、生物碱等,其中脂肪酸及其衍生物占主导地位,尤其是油酸、花生四烯酸和肾上腺酸含量在菌柄伸长区显著高于其他区段。根据金针菇菌柄的代谢物积累特点,推测脂肪酸及其衍生物可能参与调控金针菇菌柄伸长。

Unsaturated fatty acids as potential regulator involved in stipe elongation of Flammulina filiformis

Stipe is a key indicator for evaluation of the qualities of Flammulina filiformis product. The part of 0.6-1.5cm below the cap is the stipe elongation zone, and other parts are not elongate. To study the regulation mechanism of stipe elongation, LC-MS/MS was used to detect the metabolites accumulated in the elongate, transitional and non-elongate segments of the stipes, and the differential metabolites were analyzed by using statistical method such as HCA and t test. Metabolite enrichment was analyzed by KEGG. The results showed that 69 metabolites were found to be gradationally different in elongate, transitional and non-elongate segments, and the content of 52 differential metabolites was gradually reduced, and that of 17 metabolites gradually increased. The differential metabolites include organic acids, fatty acids, lipids and nucleotides, and fatty acids predominate. The content of oleic acid, arachidonic acid and adrenic acid was significantly higher in elongate part than that in other parts. It was inferred that the unsaturated fatty acids and their derivatives might take part in regulating the elongation growth of stipe of Flammulina filiforms.