蜡螟-单梗着色霉Fonsecaea monophora感染模型的构建及其血淋巴功能的变化

暗色真菌单梗着色霉Fonsecaea monophora侵犯皮肤及皮下组织,引起着色芽生菌病;其所引起的皮肤疣状改变或溃疡严重影响患者生活质量,重者可致残甚至发生癌变。近年来,无脊椎动物构建感染模型逐渐兴起,应用范围逐渐扩大。本研究利用蜡螟成功构建了F. monophora体内感染的动物模型,绘制了被感染蜡螟幼虫的生存曲线,观察记录了感染过程中蜡螟幼虫体表及体腔内的变化情况。在此基础上,进一步探索蜡螟幼虫对F. monophora的免疫防御应答,初步研究发现由病原体感染所诱发的血淋巴改变具有一定的抑真菌性,可抑制白念珠菌的生长。

Development of Galleria mellonella-Fonsecaea monophora infection model and functional changes of hemocytes in infected larvae

The fungus Fonsecaea monophora invades the skin and subcutaneous tissue, and causes chromoblastomycosis in humanity, inducing skin morphological changes or ulceration. In severe cases, it can be crippled or even cancerous, seriously affecting the quality of patients’ life. In recent years, the use of invertebrates to replace mammals to construct infection models has gradually emerged, and its application range is increasingly expanding. In this study, we successfully established an animal model of F. monophora infection in vivo using larvae of Galleria mellonella, and plotted the survival curves of infected larvae, and observed changes in the surface and body cavity of larvae during infection. The immune defense response of the wax larvae against F. monophora was further investigated. This study indicates that the changes in hemocytes induced by pathogen infection have specific antifungal properties and can inhibit the growth of Candida albicans.