蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成肾脏和睾丸损伤的恢复作用

本文用腺嘌呤建立肾脏和睾丸损伤的小鼠模型,探索蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成的肾脏和睾丸损伤的恢复作用。将小鼠随机分为空白组、模型组和蝙蝠蛾拟青霉菌Cs-4菌粉的5个剂量组（350mg/kg、550mg/kg、750mg/kg、1 500mg/kg、3 000mg/kg）,连续给予相应药物21d,对各组小鼠的肾脏、睾丸通过HE染色、Masson染色,进行组织病理学检查,测定各组小鼠血清中尿素氮（BUN）、肌酐（Cr）、促卵泡刺激素（FSH）、黄体生成素（LH）含量,测定睾丸组织匀浆中的酸性磷酸酶（ACP）、琥珀酸脱氢酶（SDH）和乳酸脱氢酶（LDH）水平。结果表明,蝙蝠蛾拟青霉菌Cs-4菌株具有使小鼠体重逐渐恢复上升趋势、使受损的肾脏和睾丸组织结构恢复的作用,能使BUN、Cr、FSH、LH、ACP、SDH和LDH水平趋于正常化。

Recovery effects of fermented powder products of Paecilomyces hepiali Cs-4 on adenine-induced kidney and testis damage

A mouse model of kidney and testis injury was established using adenine to explore the recovery effect of fermented powder products of Paecilomyces hepiali Cs-4 on adenine-induced kidney and testis damage. The mice were randomly divided into control group, model group, five treatment groups orally treated with different doses of fermented powder products of Paecilomyces hepiali Cs-4 (350mg/kg, 550mg/kg, 750mg/kg, 1 500mg/kg, 3 000mg/kg). The mice were treated daily for consecutive 21 days. The histopathological observation of the kidney and testis of each group of mice was carried out by using HE and Masson staining. The content of BUN, Cr, FSH and LH in blood serum were detected. In addition, the content of lactate dehydrogenase (LDH), acid phosphatase (ACP) and succinate dehydrogenase (SDH) in testis homogenate were detected. The results showed that the body weight of mice gradually recovered and increased in the experimental group treated with fermented powder products of Paecilomyces hepiali Cs-4, manifesting a recovery effect on damaged kidney and testis structure, and normalization of BUN, Cr, FSH, LH, ACP, SDH and LDH levels.