申克孢子丝菌Pall和Pall样蛋白生物信息学分析

为初步了解申克孢子丝菌Sporothrix schenckii基因组中真菌特有蛋白PalI和PalI样蛋白的生物学特征,利用在线生物信息学分析软件,对两蛋白理化特征、功能域、二级结构、抗原表位等进行分析预测。发现两蛋白理论分子量分别为24.6kDa和75.6kDa,等电点均偏碱性; 属于同一蛋白家族,具有相同的功能域和多个PKC磷酸化位点; 两蛋白在近氨基端区域相似度较高,并与其他模式真菌中同源蛋白有很高相似度; 同时,两蛋白都有跨膜区,氨基端序列三级结构极为相似,并有丰富的抗原表位。通过生物信息学分析对申克孢子丝菌PalI和PalI样蛋白生物学特征有了初步的认识,为后续实验研究提供有力支持。

Bioinformatics analysis of PalI and PalI-like proteins from Sporothrix schenckii

Biological characteristics of PalI and PalI-like proteins of Sporothrix schenckii acquired from GenBank were analyzed by online bioinformatics analysis software. The biological properties of these two proteins, including physical and chemical properties, functional domains, secondary structure, epitopes, etc. were predicted. The theoretical molecular weights of these two proteins were 24.6kDa and 75.6kDa and their isoelectric points were about pH 9. They had the same functional domain and belonged to the same protein family with many PKC phosphorylation sites. Amino terminal regions of these two proteins were highly similar, bearing a strong resemblance to orthologous proteins of other fungi. Transmembrane regions and epitopes were found in both proteins. 3D structures of amino terminal regions of these two proteins were highly similar. These findings provide support for future experimental study.