金针菇CA基因家族分析及其对CO2的响应

金针菇栽培通过提高栽培室CO₂浓度来抑制菌盖生长,提高商品质量。碳酸酐酶能调节CO₂/HCO₃ ^-在细胞内的平衡,它可能是响应CO₂胁迫的关键酶。本研究利用我们已完成的金针菇Flammulina filiformis的基因组测序数据,以双色蜡蘑碳酸酐酶家族基因为参照,通过本地BLAST,得到7个金针菇碳酸酐酶家族的基因,分别命名为CA-1、CA-2、CA-3、CA-4、CA-5、CA-6、CA-7。基因结构、保守结构域和系统进化分析结果均支持这7个序列是碳酸酐酶家族的编码基因。高浓度CO₂胁迫处理金针菇子实体12h,CA-1、CA-2表达量与CO₂浓度正相关,CA-5负相关,CA-3、CA-4和CA-6没有响应,CA-7无明显规律。据此推测,CA-1、CA-2和CA-5可能是金针菇响应CO₂胁迫的关键基因之一,参与调控菌盖生长发育。

CA gene superfamily and its expressions in response to CO_2 in Flammulina filiformis

The growth of the caps of cultivated Flammulina filiformis can be inhibited by increasing environmental CO₂ concentration to improve product quality. Carbonic anhydrases (CAs) regulate the intracellular balance of CO₂/HCO₃ ^-. It is speculated that CAs probably are key enzymes in response to CO₂ stress. In the study, the completed genome data of Flammulina filiformis were utilized. Using genes of Laccaria bicolor CA family as reference, 7 putative CA genes, named CA-1, CA-2, CA-3, CA-4, CA-5, CA-6 and CA-7, were obtained from Flammulina filiformis genome. Gene structure, conserved domain and phylogenetic analyses revealed that the 7 putative CAs belonged to the carbonic anhydrase family. As a result of treatment of high concentration of CO₂ for 12 hours, the expression level of CA-1 and CA-2 in Flammulina filiformis fruiting body showed positive correlation with CO₂ concentration, however, the expression level of CA-5 showed negatively correlative, and that of CA-3, CA-4 and CA-6 unchanged, while that of CA-7 irregular. Thus, it could be speculated that CA-1, CA-2 and CA-5 might be the key genes in response to CO₂ stress in Flammulina filiformis, participating in the regulation of cap growth and development.