Buffered Romanowsky Staining of Collodion Coated Sections

It has been observed that when it is necessary to coat paraffin sections with collodion to prevent loss of parts of the sections or to prevent cell shrinkage, staining with the buffered Romanowsky technic¹ is often unsatisfactory. Under these conditions the azures generally stain well while the eosinophilic structures are tinged feebly or not at all with eosin. As the omission of accelerators had been found to give the same type of result², and as this defect had been remediable by increasing either the duration of staining or the concentration of dye, as well as by the use of accelerators, it was thought that increase either of staining time or of stain concentration might remedy the defect in the present instance as well.