| Abstract: | Energized cells of Streptococcus lactis conserve and transduce energy at the plasma membrane in the form of an electrochemical gradient of hydrogen ions (Δp). An increase in energy-consuming processes, such as cation transport, would be expected to result in a change in the steady state Δp. We determined the electrical gradient (ΔΨ) from the fluorescence of a membrane potential-sensitive cyanine dye, and the chemical H+ gradient (ΔpH) from the distribution of a weak acid. In glycolyzing cells incubated at pH 5 the addition of NaCl to 200 mM partially dissipated the Δp by decreasing ΔΨ, while the ΔpH was constant. The Δp was also determined independently from the accumulation levels of thiomethyl-β-galactoside. The Δp values decreased in cell fermenting glucose at pH 5 or pH 7 when NaCl was added, while the ΔpH values were unaffected; cells fermenting arginine at pH 7 showed similar effects. Thus, these nongrowing cells cannot fully compensate for the energy demand of cation transport. |
