Engineering 2,4-D resistance into cotton |
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| Authors: | C Bayley N Trolinder C Ray M Morgan J E Quisenberry D W Ow |
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| Institution: | (1) Plant Gene Expression Center, USDA/ARS, and Department of Plant Pathology, University of California at Berkeley, 800 Buchanan St., 94710 Albany, CA, USA;(2) Cropping Systems Research Laboratory, USDA/ARS, Route 3, Box 213, 79401 Lubbock, TX, USA |
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| Abstract: | Summary To reduce damage by drift-levels of the herbicide 2,4-dichlorophenoxyacetic acid, we have engineered the 2,4-D resistance trait into cotton (Gossypium hirsutum L.). The 2,4-D monooxygenase gene tfdA from Alcaligenes eutrophus plasmid pJP5 was isolated, modified and expressed in transgenic tobacco and cotton plants. Analyses of the transgenic progeny showed stable transmission of the chimeric tfdA gene and production of active 2,4-D monooxygenase. Cotton plants obtained were tolerant to 3 times the field level of 2,4-D used for wheat, corn, sorghum and pasture crops. |
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| Keywords: | Genetic modification Gene transfer tfdA Gossypium hirsutum Herbicide |
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