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Guluronate content of alginate in Laminaria japonica and Laminaria angustata fronds in laboratory culture |
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| Authors: | Masashi Shinohara Ryuichi Nishida Makoto Nishizawa Hideo Bando Taizo Motomura Masakazu Tatewaki |
| Institution: | Kyosei Pharmaceutical Co. Ltd, 3–1–1, Megumino-kita, Eniwa 061–1374, Japan;Pharmaceutical Division, Sakai Chemical Industry Co. Ltd, 1330–1, Matsugaoka-nakamachi, Kawachinagano 586–0006, Japan;Hokkaido Pharmaceutical College, 7–1, Katsuraoka-cho, Otaru 047–0364, Japan;Institute of Algological Research, Hokkaido University, Muroran 051–0003, Japan |
| Abstract: | The guluronate (G) content of alginate in the fronds of Laminaria japonica and Laminaria angustata, cultured in the laboratory from zoospore via gametophyte using PESI medium, was determined by the 1H‐nuclear magnetic resonance spectroscopic method. The G content of alginates in young fronds cultured in various conditions were shown to exceed 55%. These values were remarkably higher than those in field kelp. The G content increased with extending culture period and at low temperature. |
| Keywords: | alginate culture guluronate content Laminaria angustata Laminaria japonica |