Measurement of the diameters of nucleoli by the template method

The method of measuring the nucleoli diameters in the light microscope is described. The nucleoli are compared to two standard templates followed by calculation of the means value and the standard deviation by means of special formulas. The scale marks of ocular micrometer or of special ocular scale can be used as the templates. The use of templates makes the measuring of nucleoli diameters 3-3.3 times as quick as the screw ocular micrometer, and with good correlation between the results obtained with both methods, too.