Immune cytolysis of human malignant melanoma by antibody to oncofetal antigen-I (OFA-I) |
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Authors: | Neil Sidell Reiko F. Irie S. David Nathanson Donald L. Morton |
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Affiliation: | (1) Division of Oncology, Department of Surgery, 54-140 CHS, UCLA School of Medicine, University of California, 90024 Los Angeles, CA, USA;(2) Surgical Service, Sepulveda Veterans Administration Hospital, 91343 Sepulveda, CA, USA |
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Abstract: | Summary IgG anti-OFA-I found in melanoma patients was tested for its ability to lyse human tumor cells in antibody-dependent cell-mediated cytotoxicity (ADCC). Sera from 89 stage II melanoma patients which contained non-HLA-related IgG antibody to an OFA-I-positive melanoma cell line (M14) as tested by indirect membrane immunofluorescence (IMI) were originally chosen as possible sources of IgG anti-OFA-I. Of those tested for specific IgG activity to OFA-I by IMI, anti-OFA-I was found only in those patients immunized with OFA-I-positive tumor cells. When the same sera were tested in ADCC, no non-HLA-related activity could be demonstrated. This result was confirmed with purified IgG fractions that could, nevertheless, show anti-OFA-I reactivity in a complement-dependent cytotoxicity assay. The fact that naturally occurring IgG anti-OFA-I antibody was not readily detectable in patients' sera and that induced IgG anti-OFA-I did not participate in ADCC indicates that OFA-I-related tumor cell lysis via ADCC is an unlikely phenomenon in cancer patients. |
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