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Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomatoes using RAPD and RFLP analyses |
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| Authors: | M R Stevens E M Lamb D D Rhoads |
| Institution: | (1) Department of Horticulture and Forestry, University of Arkansas, 72701 Fayetteville, AR, USA;(2) Department of Biological Sciences, University of Arkansas, 72701 Fayetteville, AR, USA;(3) Present address: Department of Agronomy and Horticulture, Brigham Young University, 275 Widtsoe Building, 84602 Provo, Utah, USA |
| Abstract: | The Sw-5 locus confers dominant resistance to tomato spotted wilt virus (TSWV). To map the location and facilitate the identification of markers linked to Sw-5 we developed a pair of near-isogenic lines (NILs) and an F2 Lycopersicon esculentum x L. pennellii population segregating for resistance to TSWV. DNA from the NILs was analyzed using 748 random 10-mer oligonucleotides to discern linked molecular markers using a random amplified polymorphic DNA (RAPD) approach. One random primer (GAGCACGGGA) was found to produce a RAPD band of about 2200 bp that demonstrates linkage to Sw-5. Data from co-segregation of resistance and restriction fragment length polymorphisms (RFLPs) in a F2 interspecific population position Sw-5 between the markers CT71 and CT220 near the telomere of the long arm of chromosome 9. |
| Keywords: | Lycopersicon esculentum Lycopersicon peruvianum RAPD RFLP Tomato spotted wiltvirus (TSWV) |
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