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Duration of the Mitotic Cycle in Cell Cultures of Haplopappus gracilis
Authors:Tage  Eriksson
Institution:Institute of Physiological Botany University of Uppsala, Sweden
Abstract:The duration of the cell cycle and its component phases in cell cultures of Haplopappus gracilis was estimated by means of pulse labelling with tritiated thymidine and subsequent autoradiographic techniques. The total duration of the mitotic cycle was found to be 22.0 hours. The average durations of the following component phases were: the synthetic period (S) 6.4 hours, the postsynthetic period (G2) 4.86 hours, prophase (P) 0.64 hours, metaphase (M) 0.40 hours, anaphase + early telophase (AT) 0.36 hours, the presynthetic period (G1) 9.34 hours. The results indicate that G1 and G2 are the phases, which are most prolonged in populations of cultivated cells when compared to the same phases in root lip cells from the same species.
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