Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a |
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Authors: | Raúl Teruel Irene Martínez-Martínez José A Guerrero Rocío González-Conejero María E de la Morena-Barrio Salam Salloum-Asfar Ana B Arroyo Sonia águila Nuria García-Barberá Antonia Mi?ano Vicente Vicente Javier Corral Constantino Martínez |
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Affiliation: | 1.Centro Regional de Hemodonación, University of Murcia, IMIB, Spain, C/Ronda de Garay S/N, 30003, Murcia, Spain |
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Abstract: | BackgroundDevelopmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant.ResultsIn this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control.ConclusionsThese data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes. |
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Keywords: | miRNAs Sialytransferases Antithrombin Post-translational modifications Microarray Post-natal development |
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