Arbovirus detection in synanthropic mosquitoes from the Brazilian Amazon and in mosquito saliva using Flinders Technology Associates cards |
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| Affiliation: | 1. Laboratório de Entomologia I, Fiocruz Rondônia – Fundação Oswaldo Cruz, Lagoa, 76812-245, Porto Velho, Rondônia State, Brazil;2. Programa de Pós-Graduação em Biologia Experimental – PGBIOEXP, Fundação Universidade Federal de Rondônia – UNIR, BR-364, km 9.5, 76801-059, Porto Velho, Rondônia State, Brazil;3. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia, Instituto Leônidas e Maria Deane – Fiocruz Amazônia, Adrianópolis, 69057-070, Manaus, Amazonas State, Brazil;4. Programa de Pós-Graduação em Biologia Celular e Molecular, Instituto Oswaldo Cruz, Fiocruz, Manguinhos, 21040-360, Rio de Janeiro State, Brazil;5. Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro, Instituto Leônidas e Maria Deane – Fiocruz Amazônia, Adrianópolis, 69057-070, Manaus, Amazonas State, Brazil;6. Laboratório de Virologia Molecular, Fiocruz Rondônia – Fundação Oswaldo Cruz, Lagoa, 76812-245, Porto Velho, Rondônia State, Brazil;7. Instituto Nacional de Epidemiologia da Amazônia Ocidental – INCT-EpiAmO, Lagoa, 76812-245, Porto Velho, Rondônia State, Brazil;1. Department of Pharmacy, Guangdong Second Provincial General Hospital, No.466 Middle Xingang Road, Guangzhou, 510317, China;2. Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, No. 1838 Shatai South Road, Baiyun District, Guangzhou, 510515, China;3. Department of Pharmacy, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, No.23 Haipang Street, North Street, Jiangmen, 529030, China;1. Division of Pharmacy Practice and Administration, University of Missouri-Kansas City School of Pharmacy, Kansas City, Missouri, USA;2. Department of Pharmacy, Centerpoint Medical Center, Independence, Missouri, USA;1. Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China;2. Institute of Oceanology and Marine Fisheries, Nantong, 226007, Jiangsu, China;3. Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning, 530008, Guangxi, China;1. Department of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China;2. Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China;3. Medical College, Anhui University of Science and Technology, Huainan, China;4. Department of Preventive Dentistry, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China;5. Department of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai, China;1. Institute of Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China;2. Infectious Diseases Institute, Guangzhou Eighth People''s Hospital, Guangdong, China;3. School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, China |
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| Abstract: | Although arbovirus transmission and identifying target vectors may provide a baseline for planning disease control strategies, there are many gaps in knowledge regarding these mosquitoes and viral species in urban, rural, or sylvatic habitats in the Brazilian Amazon. Our goal was to screen for dengue, chikungunya, and Zika viruses in synanthropic mosquitoes and with Flinders Technology Associates (FTA) cards using insect saliva. Mosquitoes were caught using ovitraps and aspirators in the city of Porto Velho, Rondônia, Brazil. Honey-baited FTA cards were placed in mosquito cages for 15 days; whole mosquitoes and FTA cards were analysed for viral RNA using RT-qPCR assays. One pool of Aedes aegypti females was found to be infected with the Zika virus and one male mosquito was infected with dengue-4, suggesting natural vertical/venereal transmission. Our study also reported evidence of vertical/venereal transmission of ZIKV in Culex quinquefasciatus males for the first time in the Brazilian Amazon, and the feasibility of using FTA cards to detect arboviruses in the saliva of field-collected mosquitoes. Vertical/venereal transmission of viruses by atypical mosquito species reinforces the need for combined viral and entomological screening in arbovirus surveillance programs. |
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| Keywords: | Arboviruses Phylogeny Real-time PCR |
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