红豆草耐盐愈伤组织的筛选及植株再生

将红豆草种子在含１．２％ＮａＣｌ的ＭＳ培养基上萌发以消除盐敏感的幼苗，把存活的幼苗下胚轴切段在含１ｍｇ／Ｌ２，４－Ｄ、０．５ｍｇ／Ｌ６－ＢＡ及１．２％ＮａＣｌ的ＭＳ培养基上诱导愈伤组织，通过连续筛选得到可耐受１．８％ＮａＣｌ的愈伤组织，在有０．２ｍｇ／Ｌ ＮＡＡ和１ｍｇ／Ｌ ＩＡＡ存在下该愈伤组织分化出芽，待幼，待幼苗长至３ｃｍ左右时转至含２ｍｇ／ＬＮＡＡ和或ＩＢＡ的１／２ＭＳ培养基上生根。对对照

Selection for salt tolerance in tissue culture of sainfoin and recovery of plants from NaCl tolerant callus

Seeds of sainfoin were germinated in MS medium plus 1.2% NaCl to eliminated the salt sensitive seedlings and the hypocotyl segments of survived seedlings were used to induce callus on MS medium containing 1 mg/L 2,4-dichlorophenoxyacetic acid,0.5 mg/L 6-benzyladenine and 1.2% NaCl.The organigenetic callus tolerating 1.8% NaCl was obtained by continuos selection on NaCl containing medium.Shoots regenerated from the selected callus on MS medium containing 0.2 mg/L α-naphthaleneacetic acid and 1 mg/L indole-3-butyric acid.Roots were induced from 3 cm-high shoots on half-strength MS medium plus 2 mg/L α-naphthaleneacetic acid or indole-3-butyric acid.The selected tolerant callus and the reinitiated callus from the regenerated plant showed a higher tolerance to osmotic stress than the nonselected callus.