Establishment of primate lymphoblastic cell lines by coculture with a simian T-cell leukemia virus-1 positive, hypoxanthine-guanine-phosphoribosyltransferase negative Japanese macaque cell line |
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| Authors: | Y Murayama T Ishida M Minezawa A Noguchi O Takenaka |
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| Affiliation: | Primate Research Institute, Kyoto University, Aichi, Japan. |
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| Abstract: | A cell line (JAMH17+) resistant to 8-azaguanine was established from a human T-cell leukemia virus type 1 related virus (simian T-cell leukemia virus-1) positive Japanese macaque cell line. Lymphoblastic cell lines were established from the peripheral blood mononuclear cells of humans, hominoids, and several species of macaques by coculture with JAMH17+ in hypoxanthine-aminopterin-thymidine medium. HTLV-1 specific antigen was detected in some of the established cell lines. Phenotypic analysis showed that several cell lines of crab-eating macaques expressed Leu11a antigen, which is a marker of human natural killer cells. |
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| Keywords: | Japanese macaque Simian T-cell leukemia virus-1 8-azaguanine Lymphoblastic cell lines Hominoids Crab-eating macaques Sulawesi macaques |
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