NADH-Linked oxidative phosphorylation inNitrobacter agilis

A particulate cell-free fraction (144,000-X-g pellet) fromNitrobacter agilis catalyzes the acrobic or anaerobic oxidation of NADH. Phosphorylation coupled to the aerobic oxidation of NADH yields P/O ratios of 1.1. The net formation of ATP coupled to the anaerobic oxidation of NADH by nitrate yields P/NO₃ ratios of 0.7. Phosphate esterification is uncoupled by carbonylcyanide-m-chlorophenyl-hydrozone and is sensitive to inhibitors of the electron transport system.