Selective cytotoxicity of squamocin on T24 bladder cancer cells at the S-phase via a Bax-, Bad-, and caspase-3-related pathways |
| |
Authors: | Yuan Shyng-Shiou F Chang Hsueh-Ling Chen Hsiao-Wen Kuo Fu-Chen Liaw Chih-Chuang Su Jinu-Huang Wu Yang-Chang |
| |
Institution: | Department of Medical Research, E-DA Hospital, I-Shou University, Kaoshiung County, Taiwan 824, ROC. yuanssf@ms33.hinet.net |
| |
Abstract: | Annonaceous acetogenins are a group of potential anti-neoplastic agents isolated from Annonaceae plants. We purified squamocin, a cytotoxic bis-tetrahydrofuran acetogenin, from the seeds of Annona reticulata and analyzed its biologic effects on cancer cells. We showed that squamocin was cytotoxic to all the cancer lines tested. Furthermore, squamocin arrested T24 bladder cancer cells at the G1 phase and caused a selective cytotoxicity on S-phase-enriched T24 cells. It induced the expression of Bax and Bad pro-apoptotic genes, enhanced caspase-3 activity, cleaved the functional protein of PARP and caused cell apoptosis. These results suggest that squamocin is a potentially promising anticancer compound. |
| |
Keywords: | Squamocin Annona reticulata Acetogenins Bladder cancer Apoptosis Cell cycle |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|