Structure prediction, evolution and ligand interaction of CHASE domain |
| |
Authors: | Pas Jakub von Grotthuss Marcin Wyrwicz Lucjan S Rychlewski Leszek Barciszewski Jan |
| |
Affiliation: | BioInfoBank Institute, ul. Limanowskiego 24A, 60-744 Poznan, Poland. kuba@bioinfo.pl |
| |
Abstract: | Cytokinins are plant hormones involved in the essential processes of plant growth and development. They bind with receptors known as CRE1/WOL/AHK4, AHK2, and AHK3, which possess histidine kinase activity. Recently, the sensor domain cyclases/histidine kinases associated sensory extracellular (CHASE) was identified in those proteins but little is known about its structure and interaction with ligands. Distant homology detection methods developed in our laboratory and molecular phylogeny enabled the prediction of the structure of the CHASE domain as similar to the photoactive yellow protein-like sensor domain. We have identified the active site pocket and amino acids that are involved in receptor-ligand interactions. We also show that fold evolution of cytokinin receptors is very important for a full understanding of the signal transduction mechanism in plants. |
| |
Keywords: | ACT, aspartate kinase-chorismate mutase-TyrA CACHE, Ca2+ channels and CHEmotaxis receptors CHASE, cyclases/histidine kinases associated sensory extracellular GAF, cGMP phosphodiesterase, adenyl cyclase, FhlA domain GRDB, gene related data base PAS, periodic clock protein, aryl hydrocarbon receptor and single-minded protein PDB, protein data bank PYP, photoactive yellow protein |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|