In vitro synthesis of a specific protein viz. Asparagine synthetase by nuclear fraction from gamma irradiated potato buds |
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| Authors: | P M Nair S G Shirsat |
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| Affiliation: | Biochemistry & Food Technology Division Bhabha Atomic Research Centre Trombay, Bombay 400 085, USA |
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| Abstract: | Irradiation induced synthesis of asparagine synthetase was localized in the nucleus of the potato buds. Isolated nuclei were capable of incorporating amino acids into asparagine synthetase protein and the process was inhibited by cycloheximide (100%), puromycin (73%) and by feed back inhibitor β-aspartyl hydroxamate (100%). Preincubation of the bud tissue with actinomycin D was necessary to express its inhibitory effect. |
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