Component C of the methylcoenzyme M methylreductase system contains bound 7-mercaptoheptanoylthreonine phosphate (HS-HTP) |
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| Authors: | K M Noll R S Wolfe |
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| Institution: | 1. Division of Gastroenterology and Hepatology, Hepatitis C Center, Department of Medicine, University of Colorado, Denver, Aurora, Colorado;2. Integrated Department in Immunology: University of Colorado Denver and National Jewish Health, Denver, Colorado;3. Division of Renal Diseases and Hypertension, Department of Medicine, University of Colorado Denver, Aurora, Colorado;4. Department of Immunology, University of Washington, School of Medicine, Seattle, Washington;5. Department of Pathology, Center for Innovative Regenerative Therapies, Department of Surgery, Transplantation Section, Children’s Hospital of Pittsburgh, McGowan Institute for Regenerative Medicine and the Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, Pennsylvania;6. Mechanical and Biomedical Engineering, Colorado State University, Fort Collins, Colorado;7. Department of Medicine, University of North Carolina, Chapel Hill, North Carolina;8. Department of Medicine and Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania;9. Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Denver, Aurora, Colorado;10. Mayo Clinic, Division of Gastroenterology and Hepatology, Rochester, Minnesota;11. Eastern Colorado Veteran’s Affairs Medical Center, Denver, Colorado;1. Swiss Tropical and Public Health Institute, Socinstrasse 57, P.O. Box, CH-4002 Basel, Switzerland;2. University of Basel, Petersplatz 1, CH-4001 Basel, Switzerland;3. Laboratoire Central Vétérinaire, Km 8, Route de Koulikoro, BP 2295 Bamako, Mali;4. Anses-Nancy Laboratory for Rabies and Wildlife, European Union Reference Laboratory for Rabies, WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, European Union Reference Laboratory for Rabies Serology, Technopôle agricole et vétérinaire de Pixérécourt, CS 40009, F-54220 Malzéville, France |
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| Abstract: | The structure of component B of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum was recently found to be 7-mercaptoheptanoylthreonine phosphate (HS-HTP). The work described here demonstrates that this compound is found in two forms: enzyme-free and enzyme-bound. HS-HTP was found to be bound to component C of the methylcoenzyme M methylreductase system. The cofactor extracted from the protein by heat denaturation was found to comigrate with the mixed disulfide of HS-HTP and 2-mercaptoethanol by high-performance liquid chromatography, suggesting HS-HTP is not modified in the bound state. |
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