半胱胺盐酸盐减缓山羊手术后细胞免疫抑制效应的实验研究

Cysteamine attenuates suppression of peripheral IL-2 and lymphocytic proliferation induced by ruminal and duodenal operations in goats]

Sixteen castrated male goats were randomly allocated into two groups (Control n=8; Experiment n=8) to investigate the effects of cysteamine on surgery-induced cellular immune suppression. The experiment commenced with 13 d of pre-operation (pre-Op), which contained 3 d of roughage-fed period (RP) and 10 d of roughage + concentrate-fed period (RCP), followed by first operation (Op-1) and then the second operation (Op-2). The goats were allowed to have 14 d of recovery between two operations. The fistulas were fixed to rumen walls in Op-1 and to duodenal walls in Op-2. Cysteamine (15 mg x kg(-1) x BW x d(-1)) was added to diet and fed to goats in Exp. group since RCP of pre-Op through whole experimental period. Blood was sampled through jugular vein from goats of both groups at pre-feeding of the last day of RP and RCP, 8 d after Op-1, 4 d and 13 d after Op-2, respectively to determine serum concentration of interlukin-2 (IL-2), cortisol, as well as PHA-stimulated lymphocytic proliferation rate (SI). In pre-operative periods there were no significant differences in concentration of IL-2 (0.82+/-0.1 vs 1.03+/-0.22 microg l(-1)) and cortisol (20.48+/-4.52 vs 20.80+/-9.93 microg l(-1)), as well as lymphocytic SI (90.72+/-7.25 vs 129.22+/-18.59) between Control and Exp. groups. The concentrations of IL-2 (0.82+/-0.1 vs 0.73+/-0.08 vs 0.55+/-0.12 microg l(-1)) and lymphocytic SI (90.72+/-7.25 vs 49.31+/-6.4 vs 7.09+/-1.66) in Control were depressed acutely (P<0.05), but cortisol concentration elevated (20.48+/-4.52 vs 26.67+/-10.51 vs 32.33+/-3.29 microg l(-1)) in post-operative periods (8 d after Op-1 and 4 d after Op-2), compared with those in RCP. While they remained unaltered in Exp. group except a slight decrease of lymphocytic SI at 4 d after Op-2. The different responses of Control and Exp. groups to surgery stress led to a 80-90% higher of IL-2 and about 3 times greater of SI in Exp. than those in Control, which was contributed by cysteamine administration. Our data show that in operated goats the cellular immune was suppressed by surgery stress, cysteamine administration prevented decreases of IL-2 and lymphocytic SI and increase of cortisol induced by surgery stress, consequently attenuates surgery-induced suppression of cellular immune activity.