Pentraxin 3 is an anti-inflammatory protein associated with lipid-induced interleukin 10 in vitro |
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Institution: | 1. Alpert Medical School at Brown University, Providence, RI, USA;2. Department of Radiology, Rhode Island Hospital, Providence, RI, USA;3. Department of Pathology, Rhode Island Hospital, Providence, RI, USA;4. Department of Surgery, Rhode Island Hospital, Providence, RI, USA;1. Department of Women''s and Children''s Health, Obstetrics and Gynecology, Uppsala University, Uppsala, Sweden;2. Department of Clinical Sciences, Karolinska Institutet, Danderyd Hospital, Stockholm, Sweden;3. Department of Clinical Chemistry, Medical Science, Uppsala University, Uppsala, Sweden;4. Department of Cardiology, Medical Science, Uppsala University, Uppsala, Sweden |
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Abstract: | Pentraxin 3 (PTX3) is an acute phase protein expressed in response to pro-inflammatory stimuli during atherosclerosis. However, recent findings suggest that PTX3 is a counter-regulatory protein which enhances the anti-inflammatory response.ObjectiveTherefore, the capacity of PTX3 to alter the inflammatory milieu following in vitro stimulation of PBMCs with the pro-inflammatory lipid, palmitate, was examined.MethodsPBMCs from 17 healthy male donors were isolated and cultured under four separate conditions; 200 μmol/L palmitate, a physiologically relevant concentration of PTX3, in combination (pal + PTX3), and an unstimulated time-course control.ResultsPalmitate-induced production of the counter-regulatory protein PTX3 was positively associated with the production of the anti-inflammatory cytokine interleukin 10 (IL-10) following in vitro stimulation of human PBMCs. Furthermore, stimulation of PBMCs in vitro with 500 pg/mL PTX3 elicited a significantly greater increase in IL-10 production compared to the palmitate stimulated conditions. However, PTX3 stimulation did not result in the production of the pro-inflammatory cytokines IL-1β, IL-6, and tumor necrosis factor alpha, and when combined with palmitate, did not alter the pro-inflammatory milieu from PBMCs in this study.ConclusionThese findings provide evidence supporting the role of PTX3 as a mediator of the anti-inflammatory response in physiologically relevant conditions, and suggests that PTX3 counter regulates the development of atherosclerosis by enhancing the production of IL-10. |
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Keywords: | PTX3 Palmitate Atherosclerosis Inflammatory cytokines Interleukin-10 IL"} {"#name":"keyword" "$":{"id":"k0035"} "$$":[{"#name":"text" "_":"interleukin PTX3"} {"#name":"keyword" "$":{"id":"k0045"} "$$":[{"#name":"text" "_":"pentraxin 3 PBMC"} {"#name":"keyword" "$":{"id":"k0055"} "$$":[{"#name":"text" "_":"peripheral blood mononuclear cell TLR"} {"#name":"keyword" "$":{"id":"k0065"} "$$":[{"#name":"text" "_":"Toll-like receptor TNF-α"} {"#name":"keyword" "$":{"id":"k0075"} "$$":[{"#name":"text" "_":"tumor necrosis factor-alpha |
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