Stability of cytokines,chemokines and soluble activation markers in unprocessed blood stored under different conditions |
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| Affiliation: | 1. Department of Epidemiology, UCLA, Los Angeles, CA 90095-1772, United States;2. Department of Biostatistics, UCLA, Los Angeles, CA 90095-1772, United States;3. Fielding School of Public Health, UCLA, Los Angeles, CA 90095-1772, United States;4. The Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA 90095-1713, United States;5. David Geffen School of Medicine, UCLA, Los Angeles, CA 90095-1772, United States;1. Center for Neuroscience and Department of Psychology, University of California, Davis, CA, USA;2. Cousins Center for Psychoneuroimmunology and Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, CA, USA;3. Department of Psychiatry, Stony Brook University, Stony Brook, New York, USA;4. Department of Psychology, Stony Brook University, Stony Brook, New York, USA;5. Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, New York, USA;1. Division of Surgical Oncology, Department of Surgery, David Geffen School of Medicine at University of California, Los Angeles (UCLA), Los Angeles, CA, USA;2. Department of Chemistry and Biochemistry at UCLA, Los Angeles, CA, USA;3. UCLA-DOE Institute, Los Angeles, CA, USA;4. Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA;5. The Molecular Biology Institute, UCLA, CA, USA;6. Jonsson Comprehensive Cancer Center, UCLA, CA, USA;7. UCLA AIDS Institute, Los Angeles, CA, USA;1. Department of Neurology, The Affiliated Houjie Hospital, Guangdong Medical University, PR China;2. Department of Laboratory, The Affiliated Houjie Hospital, Guangdong Medical University, PR China;3. The Community Health Service Center of Houjie Town, Dongguan City, Guangdong Province, PR China;1. Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA;1. Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey TW20 0EX, UK;2. National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK;4. School of Pharmacy and Biomedical Sciences, University of Portsmouth, White Swan Road, Portsmouth, Hants PO1 2DT, UK |
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| Abstract: | BackgroundBiomarkers such as cytokines, chemokines, and soluble activation markers can be unstable when processing of blood is delayed. The stability of various biomarkers in serum and plasma was investigated when unprocessed blood samples were stored for up to 24 h at room and refrigerator temperature.MethodsBlood was collected from 16 healthy volunteers. Unprocessed serum, EDTA and heparinized blood was stored at room (20–25 °C) and refrigerator temperature (4–8 °C) for 0.5, 2, 4, 6, 8, and 24 h after collection before centrifugation and separation of serum and plasma. Samples were batch tested for various biomarkers using commercially available immunoassays. Statistically significant changes were determined using the generalized estimating equation.ResultsIFN-γ, sIL-2Rα, sTNF-RII and β2-microglobulin were stable in unprocessed serum, EDTA and heparinized blood samples stored at either room or refrigerator temperature for up to 24 h. IL-6, TNF-α, MIP-1β and RANTES were unstable in heparinized blood at room temperature; TNF-α, and MIP-1β were unstable in unprocessed serum at room temperature; IL-12 was unstable in unprocessed serum at refrigerator temperature; and neopterin was unstable in unprocessed EDTA blood at room temperature. IL-1ra was stable only in unprocessed serum at room temperature.ConclusionAll the biomarkers studied, with the exception of IL-1ra, were stable in unprocessed EDTA blood stored at refrigerator temperature for 24 h. This indicates that blood for these biomarkers should be collected in EDTA and if delays in processing are anticipated the unseparated blood should be stored at refrigerator temperature until processing. |
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| Keywords: | Biomarker Stability Chemokines Cytokines Soluble activation marker Significant relative change |
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