Increased cold tolerance in Arabidopsis thaliana transformed with Choristoneura fumiferana glutathione S-transferase gene |
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Authors: | C Huang T Guo S C Zheng Q L Feng J H Liang and L Li |
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Institution: | (1) Guangdong Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou, 510631, P.R. China;(2) Great Lakes Forestry Center, Canadian Forest Service, Sault Ste. Marie, Ontario, P6A 2E5, Canada |
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Abstract: | A glutathione S-transferase (GST) gene cloned from the lepidopteran spruce budworm, Choristoneura fumiferana Clem. was transformed into the model plant Arabidopsis thaliana. The CfGST-transgenic and wild-type A. thaliana were subjected to 4 and 10 °C for 48 h and their cold resistance was studied. The GST activity of the transgenic plants was
46.6 and 35.7 % higher than that of the wild-type plants after 48 h under 4 and 10 °C, respectively. Relative membrane permeability
and malondialdehyde content in the transgenic plants were lower while contents of the chlorophyll and proline were higher
than those in the wild-type plants under 4 and 10 °C. The survival rate of the transgenic plants was 43.7 % for 24 h under
0 °C, while survival rate of wild-type plants was 28.3 %. The results indicated that the insect GST could enhance cold resistance
in the transgenic A. thaliana. |
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Keywords: | Additional" target="_blank">Additional chlorophyll membrane permeability malondialdehyde proline transgenic plants |
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