Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum) |
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Authors: | R. S. Patil M. R. Davey J. B. Power |
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Affiliation: | (1) Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD Nottingham, UK |
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Abstract: | Three Indian cultivars ofL. esculentum were assessed for shoot regeneration from protoplast-derived calli. Consistent yields of viable protoplasts (>9.0×106 g f.wt.-1) were obtained from leaflets of 14 days old cultured shoots. Protoplast viability (88–94%) and planting efficiency (55–70%) were recorded for the three cultivars. Up to 71% of the protoplast-derived tissues regenerated shoots.Abbreviations BAP 6-benzylaminopurine - 2,4-d 2,4-dichlorophenoxyacetic acid - g f.wt. gram fresh weight - IAA indoleacetic acid - MS Murashige & Skoog (1962) - NAA -naphthaleneacetic acid - Zea zeatin |
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Keywords: | tomato protoplasts plant regeneration |
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