CHOLINE: HIGH AFFINITY UPTAKE IN VIVO BY RAT HIPPOCAMPUS |
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| Authors: | G. M. Samaras J. F. Contrera |
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| Affiliation: | Department of Radiology, University of Maryland, School of Medicine, 660 W. Redwood Street, Baltimore, MD 21201;Department of Zoology, University of Maryland, College Park, MD, U.S.A. |
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| Abstract: | Abstract— [3H]Choline uptake has been measured in vivo in the rat hippocampus. Pharmacological agents and lesions which profoundly affect sodium-dependent, high-affinity [3H]choline uptake in vivo similarly affect [3H]choline uptake measured in vitro. Pentobarbital (65 mg/kg) and oxotremorine (0.75 mg kg) cause a decrease in [3H]choline uptake. Scopolamine (5 mg/kg) and iontophoretically applied extracellular potassium cause an increase in [3H]choline uptake. Septal lesions cause a decrease in [3H]choline uptake. Application of the general method may allow direct examination of presynaptic function and neural integration in the undisrupted living mammalian brain. |
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