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Use of surface response methodology to describe biomass production of Bifidobacterium infantis in complex media
Authors:A Azaola  P Bustamante  S Huerta  G Saucedo  R González  C Ramos  S Saval
Institution:(1) Departamento Sistemas Biológicos, Planta Piloto, Universidad Autónoma Metropolitana, A.P. 23-161, México, 16000. D.F;(2) Instituto de Ingeniería, Universidad Nacional Autónoma de México, Mexico
Abstract:An optimized medium containing Trypticasein, phytone, yeast extract and glucose is proposed to improve growth of bifidobacteria with high yeast extract concentration and decreased amounts of Trypticasein and phytone. These new growth media overcome nutritional limitations by the type and amount of amino acids contained in these sources and produced an increase from 1.8 to more than 4 g cell mass l–1.
Keywords:bifidobacteria  complex substrates  factorial design  biomass production  surface response
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