Detection of surface differences between two closely related cell populations by partitioning. Erythrocytes from inbred and out-bred rats and rat strains |
| |
| Institution: | 1. Laboratory of Chemical Biology, Veterans Administration Medical Center, Long Beach, CA 90822 U.S.A.;2. Department of Physiology and Biophysics, University of California, Irvine, CA 92717 U.S.A.;1. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey;2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Marmara University, İstanbul, Turkey;3. Department of Biochemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey;1. eHealth, CSIRO Health and Biosecurity, Floreat, WA, Australia;2. eHealth, CSIRO Health and Biosecurity, Herston, QLD, Australia;3. Department of Nuclear Medicine and Centre for PET, Austin Health, Heidelberg, VIC, Australia;4. ARC Centre of Excellence in Cognition and its Disorders, Department of Psychology, Macquarie University, North Ryde, NSW, Australia;5. Centre of Excellence for Alzheimer''s Disease Research & Care, School of Medical Sciences, Edith Cowan University, Joondalup, WA, Australia;6. School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Bentley, WA, Australia;7. Cogstate, Melbourne, VIC, Australia;8. Academic Unit for Psychiatry of Old Age, Department of Psychiatry, University of Melbourne, Parkville, VIC, Australia;9. Sir James McCusker Alzheimer''s Disease Research Unit (Hollywood Private Hospital), Perth, WA, Australia;10. Florey Institute, University of Melbourne, Parkville, VIC, Australia;11. Department of Medicine, Austin Health, University of Melbourne, Heidelberg, VIC, Australia;1. Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain;2. Servicio de Alergia, Instituto de Investigación Sanitaria-Hospital Universitario de La Princesa, 28006 Madrid, Spain;3. Department of Immunology, Max Planck Institut für Infektionsbiologie, Chariteplatz 1, 10117 Berlin, Germany;4. Department of Molecular and Cell Biology, Centre for Biodiscovery and Molecular Development of Therapeutics, James Cook University, 4811, Australia;1. Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, PR China;2. Center for Developmental Therapeutics, Seattle Children’s Research Institute, Division of Gastroenterology and Hepatology, Department of Pediatrics, University of Washington School of Medicine, Seattle, WA 98101, USA |
| |
| Abstract: | We have recently developed a new and powerful method capable of detecting, by purely physical means, surface differences between closely related red (or other) cell populations. The procedure consists of isotopically labeling (with 51Cr]chromate) aliquots of red blood cell populations. Such labeled cells are mixed with an excess of unlabeled red cells to which they are to be compared. The mixtures are subjected to countercurrent distribution in either a charge-sensitive or a non-charge-sensitive dextran-poly(ethylene glycol) aqueous phase system. The distribution curves are analyzed for total cells (in terms of hemoglobin absorbance) and labeled cells (in terms of cpm). Changes in the relative specific activities through the distribution curves are indicative of subtle differences in surface properties between such cell populations. Using this method we have found that erythrocytes from arbitrarily chosen (presumably hematologically normal) individuals differ. In the current work we have examined the surface properties of erythrocytes from Sprague-Dawley and from Lewis rats. This was done with a view to determining whether (a) differences of the type found between different humans can also be detected in other species and (b), if such differences do exist, to examine, by study of the highly inbred Lewis rat strain, whether the differences appear to have a genetic or an acquired basis. It was found that the surface properties of erythrocytes from Lewis and Sprague-Dawley rats differ as do erythrocytes among rats of the Sprague-Dawley strain. No difference was found between red blood cells from different rats of the inbred Lewis strain. These results indicate that the surface differences between red blood cells from different rats detected by partitioning have a genetic rather than acquired origin. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
