Studies on the cytochrome b6/f complex. I. Characterization of the complex subunits in Chlamydomonas reinhardtii |
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| Affiliation: | 1. Institute of Entomology, Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Guizhou University, 550025, Guiyang, People’s Republic of China;2. College of Tobacco Science, Guizhou University, 550025, Guiyang, People’s Republic of China;3. College of Environment and Life Sciences, Kaili University, 556011, Kaili, People’s Republic of China;1. School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, 225009, Yangzhou, China;2. Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China;1. The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;2. Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Debrecen, Nagyerdei krt 98, 4032 Debrecen, Hungary |
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| Abstract: | We have analyzed the heme-associated peroxidase activity in thylakoid membranes from the green algae Chlamydomonas reinhardtii after electrophoresis in the presence of sodium dodecyl sulfate. Besides cytochrome f and cytochrome b6, we observed peroxidase activity in two other bands, of 34 and 11 kDa, of unknown origin. Characterization of the b6/f complex subunits was undertaken by means of a comparison of the polypeptide deficiencies in several b6/f mutants with the polypeptide content of preparations enriched in b6/f complexes. We conclude that the b6/f complex consists of five subunits. Using site-specific translation inhibitors, we show that cytochrome f, cytochrome b6 and subunit IV are of chloroplast origin, whereas the Rieske protein and probably subunit V are translated on cytoplasmic ribosomes. A model of assembly of the complex is proposed: a cytochrome moiety, comprising the subunits of chloroplast origin, is assembled in the thylakoid membranes prior to the insertion and assembly of the subunits encoded in the nuclear genome. |
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