Relation between lipid polymorphism and transbilayer movement of lipid in rat liver microsomes |
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| Institution: | 1. Biophysics, Institute of Molecular Bioscience (IMB), NAWI Graz, University of Graz, Humboldtstr. 50/III, Graz 8010, Austria;2. BioTechMed Graz, Graz, Austria;3. Field of Excellence BioHealth – University of Graz, Graz, Austria;1. Biological Sciences, Kent State University, PO Box 5190, 44242 Kent, OH, USA;2. University of Amsterdam, Swammerdam Institute for Life Sciences, Section of Plant Physiology, Postbus 94215, 1090, GE, Amsterdam, The Netherlands;3. University of Amsterdam, Swammerdam Institute for Life Sciences, Section of Plant Cell Biology, Postbus 94215, 1090 GE Amsterdam, The Netherlands |
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| Abstract: | We have studied the effects of trinitrophenylation on the transbilayer movement of phosphatidylcholine and the macroscopic lipid structure in rat liver microsomal membranes. The transbilayer movement of phosphatidylcholine was investigated using the PC-specific transfer protein. 31P-NMR was employed to monitor the phospholipid organization in intact microsomal vesicles. The results indicate that modification of microsomes with trinitrobenzenesulfonic acid enhances the transbilayer movement of phosphatidylcholine at 4°C. Furthermore, phosphatidylethanolamine headgroup trinitrophenylation in microsomes increases the isotropic component in the 31P-NMR spectra even at 4°C, possibly representing the appearance of intermediate non-bilayer lipid structures. The observed parallel between these data suggests that phosphatidylethanolamine molecules in the microsomal membrane, probably in combination with a protein component, are able to destabilize the bilayer organization, thereby facilitating the transmembrane movement of phospholipids. |
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