Studies on photosynthetic inorganic pyrophosphate formation in Rhodospirillum rubrum chromatophores |
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| Affiliation: | 1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China;2. Haihe Laboratory of Sustainable Chemical Transformations, Tianjin 300192, PR China;3. Tianjin Key Lab Composite & Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China;4. State Key Laboratory of Chemical Engineering and Collaborative Innovation Center of Chemical Science and Engineering Tianjin, Tianjin University, Tianjin 300072, PR China;5. Tianjin Key Laboratory of Chemical Process Safety and Equipment Technology, Tianjin University, Tianjin 300072, PR China;1. College of Life Science, Tianjin Normal University, Tianjin, 300387, China;2. Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, 300387, PR China |
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| Abstract: | Photosynthetic formation of inorganic pyrophosphate (PPi) in Rhodospirillum rubrum chromatophores has been studied utilizing a new and sensitive method for continuous monitoring of PPi synthesis. Studies of the reaction kinetics under a variety of conditions, e.g., at different substrate concentrations and different electron-transport rates, have been performed. At very low light intensities the rate of PPi synthesis is twice the rate of ATP synthesis. Antimycin A, at a concentration which strongly inhibited the photosynthetic ATP formation, inhibited the PPi synthesis much less. Even at low rates of electron transport a significant rate of PPi synthesis is obtained. The rate of photosynthetic ATP formation is stimulated up to 20% when PPi synthesis is inhibited. It is shown that PPi synthesis and ATP synthesis compete with each other. No inhibition of pyrophosphatase activity is observed at high carbonyl cyanide p-trifluoromethoxyhydrazone concentration while ATPase activity is strongly inhibited under the same conditions. |
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