Osmotic swelling of unilamellar vesicles by the stopped-flow light scattering method. Elastic properties of vesicles |
| |
| Institution: | 1. Department of Pharmacology and Toxicology, College of Pharmacy and UA Cancer Center, University of Arizona, Tucson, AZ, United States of America;2. Department of Immunobiology, College of Medicine, University of Arizona, Tucson, AZ, United States of America;1. School of Environment, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand;2. CONICET and Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Instituto de Recursos Minerales (INREMI), Calle 64 y 120, La Plata 1900, Argentina;3. U.S. Geological Survey, 345 Middlefield Road, Menlo Park, CA 94025, USA;4. U.S. Geological Survey, Mackay School of Earth Sciences and Engineering, MS-176, University of Nevada, Reno, NV 89557, USA;5. Panterra Geoservices Inc., 14180 Greencrest Drive Surrey, B.C. V4P 1L9, Canada |
| |
| Abstract: | Unilamellar vesicles of l-α-dimyristoylphophatidylcholine have been prepared by the ether injection technique. Gel filtration on Sephacryl S1000 was used to obtain fractions of narrow polydispersity, of radius from 300 to 600 Å. Dynamic light scattering was used to determine the change in size of these vesicles in response to an osmotic pressure drop, and its dependence on vesicle size. The amplitude of swelling (ΔR/R) is linearly proportional to the osmotic pressure difference across the bilayer. We have determined the elastic area stretching modulus using a theory of membrane elasticity: it depends on the vesicle radius in the range of size studied. Vesicles having radius smaller than 400 Å show little or no swelling. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
