Studies on the cytochrome b6/f complex. II. Localization of the complex in the thylakoid membranes from spinach and Chlamydomonas reinhardtii by immunocytochemistry and freeze-fracture analysis of b6/f mutants |
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| Affiliation: | 1. Laboratoire de Microscopie Electronique, Institut Jacques Monod du CNRS, Université Paris VII, 2 Place Jussieu, 75251 Paris Cedex 05 France;2. Service de Photosynthèse, Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75231 Paris Cedex 05 France;1. Plant Breeding, Wageningen University & Research, P.O. Box 386, 6700 AJ Wageningen, the Netherlands;2. Division of Biochemistry, Department of Biology, University of Erlangen-Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany;3. Inner Mongolia Potato Engineering & Technology Research Centre, Inner Mongolia University, West College Road 235, Hohhot 010021, China;1. Shanghai Center for Plant Stress Biology, CAS Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China;2. Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA;3. Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA;1. CNRS, LCPO, UMR 5629, F-33600 Pessac, France;2. Univ. Bordeaux, LCPO, UMR 5629, F-33600 Pessac, France;3. CNRS, CBMN, UMR 5248, F-33600 Pessac, France;4. Univ. Bordeaux, CBMN, UMR 5248, F-33600 Pessac, France;1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;2. College of Plant Protection, Southwest University, Chongqing 400716, China;3. Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh;4. Fareed Biodiversity Conservation Center, Department of Agricultural Engineering, Khawaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan |
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| Abstract: | The distribution of the b6/f complex among stacked and unstacked thylakoid membranes was studied by immunocytochemistry and freeze-fracture analysis of mutants of Chlamydomonas reinhardtii lacking the complex. Immunogold labeling demonstrates the presence of b6/f complex in both regions of the thylakoid membrane in spinach and in C. reinhardtii. Numerous modifications were observed in the ultrastructure of the thylakoid membranes of mutants from C. reinhardtii lacking the complex. These modifications are consistent with the presence of b6/f complexes in different states of association in the stacked and unstacked regions of the thylakoid membrane. In particular we present evidence for an association of some b6/f complexes with the reaction centers of Photosystem I and II in large PFu and EFs particles, respectively. |
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