Effects of monovalent cations on derepression of phosphate transport in yeast |
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| Institution: | 1. Institute of Packaging Engineering, Shanghai Ocean University, Shanghai 201306, China;2. Colleague of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;3. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;1. Academy of Scientific and Innovative Research (AcSIR), CSIR-NCL, Pune, India;2. CSIR-National Chemical Laboratory, Pune, India;3. CSIR-Central Electrochemical Research Institute, CSIR Madras Complex, Chennai, India;4. Reliance Industries Ltd, Navi Mumbai, India;1. Politecnico di Milano School of Management, Via Raffaele Lambruschini 4, 20156 Milan, Italy;2. EADA Business School, Carrer d''Aragó, 204, 08011 Barcelona, Spain;1. Duquesne University School of Nursing, Pittsburgh, Pennsylvania;2. Western Psychiatric Institute and Clinic, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania;3. Department of Minimally Invasive Bariatric and General Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania;4. Department of Statistics, University of Pittsburgh, Pittsburgh, Pennsylvania |
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| Abstract: | The effect of monovalent cations on derepression of phosphate transport was studied. It was found that ammonium, K+ and Rb+ accelerate the derepression of phosphate transport produced by glucose in yeast (Saccharomyces cerevisiae). Na+ and Li+ were ineffective in accelerating derepression; Cs+ produced only a minor stimulation. The concentration range of both K+ and NH4+ that accelerated derepression was similar to that required for transport to occur. In the case of ammonium, the effects seem to depend exclusively on the so-called low-affinity transport system. The effect was strongly dependent on pH, with an optimum around 6; however, the increase in the pH of the medium did not produce in itself a high increase of the depression. Derepression was dependent on the presence of glucose, and it was very low with ethanol as substrate. The mechanism seems to depend on the ability that both K+ and NH4+ have to decrease the membrane potential of the cell while transported, and not on the capacity to produce the alkalinization of the cell interior. In addition, the phenomenon depends on the presence of glucose as substrate, which indicates the involvement of some product of glucose metabolism in the mechanism, and possibly some relation to catabolic repression. |
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