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Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes
Authors:S.?Agarwal,R.?K.?Sairam  author-information"  >  author-information__contact u-icon-before"  >  mailto:rks_ppl@yahoo.co.uk"   title="  rks_ppl@yahoo.co.uk"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author,G.?C.?Srivastava,R.?C.?Meena
Affiliation:(1) Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012, India
Abstract:Abscisic acid (ABA) and salicylic acid (SA) were sprayed on leaves of wheat genotypes C 306 and Hira at 25 and 40 d after sowing under moderate water stress (−0.8 MPa) imposed by adding PEG-6000 in nutrient solution. ABA and SA increased the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in comparison to unsprayed control plants. Both ABA and SA treatments decreased the contents of hydrogen peroxide and thiobarbituric acid reactive substances, a measure of lipid peroxidation, compared to unsprayed plants. The beneficial effect of increase in antioxidant enzymes activity and decrease in oxidative stress was reflected in increase in chlorophyll and carotenoid contents, relative water content, membrane stability index, leaf area and total biomass over control plants. The lower concentrations of ABA (0.5 mM) and SA (1.0 mM) were generally more effective than higher concentrations.
Keywords:ascorbate peroxidase  catalase  glutathione reductase  hydrogen peroxide  lipid peroxidation  membrane stability index  superoxide dismutase  Triticum aestivum
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