Beta-phenylpyruvate and glucose uptake in isolated mouse soleus muscle and cultured C2C12 muscle cells |
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Authors: | Ben-Abraham Ron Gazit Vered Vofsi Oded Ben-Shlomo Izahar Reznick Abraham Z Katz Yeshayahu |
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Affiliation: | Department of Anesthesiology, Tel Aviv Sourasky Medical Center, 64239 Tel Aviv, Israel. |
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Abstract: | Previous investigation demonstrated the potential of beta-phenylpyruvate at high concentration to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose-lowering mechanism, glucose uptake, and quantity of glucose transporters (GLUT1 and GLUT4) in mouse soleus muscle and C2C12 muscle cell lines were investigated following incubation with beta-phenylpyruvate in various concentrations. A marked enhancement of glucose uptake was demonstrated that peaked at 0.5 and 1.0 mM beta-phenylpyruvate in soleus muscle (P<0.01) and C2C12 cells (P<0.001), respectively. Kinetic analysis in C2C12 cells showed a twofold increase in Vmax compared with controls (P<0.001). In addition, both GLUT1 and GLUT4 levels were increased following exposure to beta-phenylpyruvate. Our findings point to a peripheral hypoglycemic effect of beta-phenylpyruvate. |
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Keywords: | phenylpyruvic acid glucose skeletal muscle cultured cells phenylketonurias glucose transporters |
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