高糖对线粒体乙醛脱氢酶2在大鼠心肌成纤维细胞中表达的影响

目的：观察心肌成纤维细胞是否存在线粒体乙醛脱氢酶2（ALDH2）的表达，探讨ALDH2在高糖诱导的心肌成纤维细胞引起纤维化发生中的作用。方法：原代培养心肌成纤维细胞，分为正常对照组（5.5 mmol/L）、正常+ALDH2激动剂Alda-1（20μmol/L）组、高糖组（30 mmol/L）、高糖+ Alda-1组。免疫荧光鉴定心肌成纤维细胞。各组细胞分别培养48 h后应用MTT法检测成纤维细胞增殖活力，RT-PCR和Western blot检测ALDH2 mRNA及蛋白的表达。结果：RT-PCR和Western blot结果显示心肌成纤维细胞ALDH2 mRNA和蛋白均有表达。与正常对照组相比，高糖组心肌成纤维细胞增殖能力提高（P < 0.01），ALDH2蛋白表达下降（P < 0.05）；与高糖组相比，高糖+ Alda-1组心肌成纤维细胞增殖能力降低（P < 0.01），ALDH2的蛋白表达增加（P < 0.05）。结论：心肌成纤维细胞存在ALDH2的表达，ALDH2激动剂Alda-1提高ALDH2的表达后可以抑制高糖引起的心肌成纤维细胞的增殖。

Effect of high concentration glucose on the expression of ALDH2 in cardiac fibroblasts of rats

Objective: To observe whether acetaldehyde dehydrogenase 2 (ALDH2) is expressed in cardiac fibroblasts and investigate the change of ALDH2 in cardiac fibroblasts when cultured with high concentration of glucose. Methods: Cultured cardiac fibroblasts were randomly divided into four groups:normal control group (5.5 mmol/L glucose), Alda-1 (the agonist of ALDH2, 20μmol/L) group, high glucose group (30 mmol/L glucose) and high glucose + Alda-1 group. Cardiac fibroblasts were identified by immunofluore-scence technique. Cell prolifera-tion was detected by MTT method after treated with drugs for 48 hours. mRNA and protein expressions of ALDH2 were determined by RT-PCR and Western blot, aimed to ensure whether ALDH2 was expressed in cardiac fibroblasts. The changes of ALDH2 protein expression in cardiac fibroblasts were tested by Western blot. Results: RT-PCR and Western blot results revealed that ALDH2 was expressed in cardiac fibroblasts. Compared with normal control group, cardiac fibroblasts proliferation was increased (P < 0.05), while the protein expression of ALDH2 was reduced (P < 0.05) in high glucose group. When treated with Alda-1, the proliferation of cardiac fibroblasts was decreased (P < 0.01), while the protein expression of ALDH2 was increased (P < 0.05) in high glucose group. Conclusion: ALDH2 was expressed in cardiac fi-broblasts. Alda-1, the agonist of ALDH2 enhanced the expression of ALDH2 and inhibited the proliferation of cardiac fibroblasts when cultured with high concentration of glucose.