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高糖对线粒体乙醛脱氢酶2在大鼠心肌成纤维细胞中表达的影响
引用本文:谷小雨,方婷婷,高琴,康品方,李正红,程向阳.高糖对线粒体乙醛脱氢酶2在大鼠心肌成纤维细胞中表达的影响[J].中国应用生理学杂志,2017,33(3):267-270.
作者姓名:谷小雨  方婷婷  高琴  康品方  李正红  程向阳
作者单位:1. 蚌埠医学院第一附属医院麻醉科, 安徽 蚌埠 233004; 2. 蚌埠医学院第一附属医院心血管内科, 安徽 蚌埠 233004; 3. 蚌埠医学院生理学教研室, 安徽 蚌埠 233030
基金项目:国家自然科学基金资助项目(81550036);安徽省自然科学基金资助项目(1508085MH169,1508085MH170);蚌埠医学院研究生科研创新计划项目(Byycx1548)
摘    要:目的:观察心肌成纤维细胞是否存在线粒体乙醛脱氢酶2(ALDH2)的表达,探讨ALDH2在高糖诱导的心肌成纤维细胞引起纤维化发生中的作用。方法:原代培养心肌成纤维细胞,分为正常对照组(5.5 mmol/L)、正常+ALDH2激动剂Alda-1(20μmol/L)组、高糖组(30 mmol/L)、高糖+ Alda-1组。免疫荧光鉴定心肌成纤维细胞。各组细胞分别培养48 h后应用MTT法检测成纤维细胞增殖活力,RT-PCR和Western blot检测ALDH2 mRNA及蛋白的表达。结果:RT-PCR和Western blot结果显示心肌成纤维细胞ALDH2 mRNA和蛋白均有表达。与正常对照组相比,高糖组心肌成纤维细胞增殖能力提高(P < 0.01),ALDH2蛋白表达下降(P < 0.05);与高糖组相比,高糖+ Alda-1组心肌成纤维细胞增殖能力降低(P < 0.01),ALDH2的蛋白表达增加(P < 0.05)。结论:心肌成纤维细胞存在ALDH2的表达,ALDH2激动剂Alda-1提高ALDH2的表达后可以抑制高糖引起的心肌成纤维细胞的增殖。

关 键 词:心肌成纤维细胞  乙醛脱氢酶2  高糖  Alda-1  大鼠  
收稿时间:2016-09-13

Effect of high concentration glucose on the expression of ALDH2 in cardiac fibroblasts of rats
GU Xiao-yu,FANG Ting-ting,GAO Qin,KANG Pin-fang,LI Zheng-hong,CHENG Xiang-yang.Effect of high concentration glucose on the expression of ALDH2 in cardiac fibroblasts of rats[J].Chinese Journal of Applied Physiology,2017,33(3):267-270.
Authors:GU Xiao-yu  FANG Ting-ting  GAO Qin  KANG Pin-fang  LI Zheng-hong  CHENG Xiang-yang
Institution:1. Department of Anesthesiology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004; 2. Department of Cardiovascular Medicine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004; 3. Department of Physiology, Bengbu Medical College, Bengbu 233030, China
Abstract:Objective: To observe whether acetaldehyde dehydrogenase 2 (ALDH2) is expressed in cardiac fibroblasts and investigate the change of ALDH2 in cardiac fibroblasts when cultured with high concentration of glucose. Methods: Cultured cardiac fibroblasts were randomly divided into four groups:normal control group (5.5 mmol/L glucose), Alda-1 (the agonist of ALDH2, 20μmol/L) group, high glucose group (30 mmol/L glucose) and high glucose + Alda-1 group. Cardiac fibroblasts were identified by immunofluore-scence technique. Cell prolifera-tion was detected by MTT method after treated with drugs for 48 hours. mRNA and protein expressions of ALDH2 were determined by RT-PCR and Western blot, aimed to ensure whether ALDH2 was expressed in cardiac fibroblasts. The changes of ALDH2 protein expression in cardiac fibroblasts were tested by Western blot. Results: RT-PCR and Western blot results revealed that ALDH2 was expressed in cardiac fibroblasts. Compared with normal control group, cardiac fibroblasts proliferation was increased (P < 0.05), while the protein expression of ALDH2 was reduced (P < 0.05) in high glucose group. When treated with Alda-1, the proliferation of cardiac fibroblasts was decreased (P < 0.01), while the protein expression of ALDH2 was increased (P < 0.05) in high glucose group. Conclusion: ALDH2 was expressed in cardiac fi-broblasts. Alda-1, the agonist of ALDH2 enhanced the expression of ALDH2 and inhibited the proliferation of cardiac fibroblasts when cultured with high concentration of glucose.
Keywords:cardiac fibroblasts  acetaldehyde dehydrogenase 2  high concentration of glucose  Alda-1  rat  
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