在生理学研究中如何严格遵循试验设计四原则

目的：为从事生理学研究的科研工作者严格遵循试验设计四原则（以下简称四原则）提供一些有效的技术方法。方法：通过简单介绍"四原则的概念"、举例阐述"生理学研究中在落实四原则方面出现的常见错误和案例"和简单介绍"生理学研究中如何遵循四原则"来阐述遵循四原则的具体做法。结果：需要根据实际情况,选择合适的随机化方法,分层随机化是常用的好方法;应力求设置合理的对照组,才能真正体现出对照原则的价值;有根据地给出每个小组合适的样本含量,是试验结果具有重现性的基本保证;严格遵循随机、对照和重复原则,最终才能实现对比组之间达到极好的均衡效果,这说明均衡原则是一个把关的原则。结论：在生理学科研中,严格遵循四原则对保证生理学研究结果准确可靠至关重要。

How to follow the four principles of experimental design in physiological research

Objective: To offer a series of efficient methods to physiological researchers in following the four principles of experimental de-sign (hereinafter referred to as four principles).Methods: We expounded the concrete way of following the four principles through giving an outline of the four principles, elaborating the common mistakes and cases in the applications of four principles in physiological research and in-troducing briefly how to follow the four principles in physiological research.Results: We should choose the appropriate randomization method according to the actual situation, usually stratified randomization was a good way. We should strive to set reasonable control group to reflect the value of control principle veritably. Giving the appropriate sample size for each group rightly was the basic guarantee for the research result could be reproduced. Following the principles of randomized, controlled and repetition strictly could achieve an excellent balance between con-trol groups ultimately, that was to say that the balanced principle was a principle of gatekeeper.Conclusion: Following the four principles strictly is critically important to assure the result accurately and reliably in physiological research.