不同强度运动结合白藜芦醇对老年肥胖大鼠RBP4的影响

目的：探讨不同强度运动结合白藜芦醇对老年肥胖大鼠内脏脂肪组织视黄醇结合蛋白4（RBP4） mRNA蛋白表达及血浆RBP4浓度的影响。方法：选择鼠龄3周的雄性SD大鼠80只，随机分为对照组和实验组：对照组（C）饲喂6.0%脂肪的普通饲料（n=12）；实验组分3个阶段饲喂36%~40%高脂饲料（n=68）。建立老年肥胖大鼠模型，选取24只建模成功的肥胖大鼠随机分为4组（n=6）：肥胖对照组（CO）、白藜芦醇组（RO）、低强度运动+白藜芦醇组（LRO）、中强度运动+白藜芦醇组（MRO）。LRO组和MRO组的运动强度分别为（12 m/min×15 min）和（15 m/min×15 min），每天运动60 min；补充白藜芦醇各组52.5 mg/kg·d灌胃1次，对照组采用等量的纯净水灌胃，持续干预8周。8周后采血和肾周、睾周、血管及内脏脂肪组织，检测血糖和血浆RBP4浓度、计算胰岛素敏感性（ISI），检测RBP4 mRNA和蛋白表达。结果：与正常组比较，模型组大鼠RBP4 mRNA和蛋白表达、血浆浓度及血糖指标明显升高（P<0.05，P<0.01），ISI明显降低（P<0.05）；与模型组比较，RO、LRO组和MRO组大鼠RBP4 mRNA和蛋白表达、血浆浓度及血糖指标明显降低（P<0.05，P<0.01），ISI明显升高（P<0.05）；RO、LRO组和MRO组之间比较，MRO组大鼠RBP4 mRNA和蛋白表达、血浆浓度及血糖指标明显降低，ISI明显升高，但无显著差异。结论：不同强度运动结合白藜芦醇能降低老年肥胖大鼠内脏脂肪组织RBP4 mRNA和蛋白表达及血浆RBP4浓度，受运动强度影响较小。

Effects of different intensities exercise combined with resveratrol on RBP4 in aged obese rats

Objective: To investigate the effects of different intensity exercise combined with resveratrol on retinol binding protein 4(RBP4) mRNA and protein expression in visceral adipose tissue and plasma RBP4 concentration of aged obese rats.Methods: Eighty male SD rats aged 3 weeks were randomly divided into the control group and the experimental group. Rats of the control group(C) were fed with ordinary feed of 6% fat (n=12). The experimental group consisted of 68 rats, which were fed with 36%~40% high fat feed in three stages. The rat model of elderly obesity was established successfully. Twenty-four obese rats were selected and randomly divided into 4 groups, including obese group (CO), resveratrol obese group (RO), low intensity exercise combined with resveratrol group (LRO) and moderate intensity exercise combined with resveratrol group (MRO). There were 6 rats in each group. The exercise intensities of the LRO group and MRO group were (12 m/min×15 min) and (15 m/min×15 min). Every day was exercised for 60 minutes. The rats in RO, LRO or MRO were treated with resveratrol at the dose of 52.5 mg/kg·d, while the rats in the control group were fed with the same amount of purified water, continued intervention for 8 weeks. After 8 weeks, samples of blood, adipose tissue of around the kidney, testicle, vessel and visceral were collected. Blood glucose and plasma RBP4 concentration were measured. Insulin sensitivity(ISI) was calculated. The expressions of RBP4 mRNA and protein were also determined.Results: Compared with normal (C) group, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the model (CO) group were increased obviously (P<0.05, P<0.01). ISI was decreased obviously (P<0.05). Compared with model (CO) group, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the RO and LRO and MRO groups were decreased significantly(P<0.05, P<0.01), while ISI was increased markedly (P<0.05). Compared with the RO and LRO groups, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the MRO group were decreased, while ISI was increased, but there was no significant difference.Conclusion: Different intensities exercise combined with resveratrol could reduce the RBP4 mRNA and protein expression in visceral adipose tissue and plasma RBP4 concentrations of aged obese rats, but less affected by exercise intensity.