The purification of human serum 1 -antitrypsin by affinity chromatography on concanavalin A |
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Authors: | I E Liener O R Garrison Z Pravda |
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Affiliation: | Department of Biochemistry College of Biological Sciences University of Minnesota St. Paul, Minnesota 55101 USA |
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Abstract: | α1-Antitrypsin (α1-AT) has been isolated from human serum by a two-step procedure which involves chromatography on DEAE-Sephadex followed by affinity chromatography on insolubilized concanavalin A. This protein appeared to be homogeneous when examined by electrophoresis on cellulose acetate and double immunodiffusion; minor contaminants, however, were detected by gel electrophoresis and immunoelectrophoresis. This procedure is readily adaptable to the large-scale purification of α1-AT and should facilitate further studies on the physicochemical and biological properties of α1-AT and its genetic variants. |
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