Gtl2
lacZ,an insertional mutation on mouse Chromosome 12 with parental origin-dependent phenotype |
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Authors: | K Schuster-Gossler D Simon-Chazottes J -L Guénet J Zachgo A Gossler |
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Institution: | 1. The Jackson Laboratory, 600 Main Street, 04609, Bar Harbor, Maine, USA 2. Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France 3. Max-Delbrück-Laboratorium in der MPG, Carl-von-Linné-Weg 10, D-50829, K?ln, Germany
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Abstract: | We have produced a transgenic mouse line, Gtl2
lacZ
(Gene trap locus 2), that carries an insertional mutation with a dominant modified pattern of inheritance:heterozygous Gtl2
lacZ
mice that inherited the transgene from the father show a proportionate dwarfism phenotype, whereas the penetrance and expressivity
of the phenotype is strongly reduced in Gtl2
lacZ
mice that inherited the transgene from the mother. On a mixed genetic background this pattern of inheritance was reversible
upon transmission of the transgene through the germ line of the opposite sex. On a predominantly 129/Sv genetic background,
however, transgene passage through the female germ line modified the transgene effect, such that the penetrance of the mutation
was drastically reduced and the phenotype was no longer obvious after subsequent male germ line transmission. Expression of
the transgene, however, was neither affected by genetic background nor by parental legacy. Gtl2
lacZ
maps to mouse Chromosome 12 in a region that displays imprinting effects associated with maternal and paternal disomy. Our
results suggest that the transgene insertion in Gtl2
lacZ
mice affects an endogenous gene(s) required for fetal and postnatal growth and that this gene(s) is predominantly paternally
expressed.
Received: 30 May 1995 / Accepted: 7 August 1995 |
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Keywords: | |
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